Neuroprotective effect of olive oil in the hippocampus CA1 neurons following ischemia: Reperfusion in mice

M Zamani; J Hassanshahi; M Soleimani; F Zamani

doi:10.4103/0976-3147.112753

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Original Article

4 (

); 164-170

doi:

10.4103/0976-3147.112753

Neuroprotective effect of olive oil in the hippocampus CA1 neurons following ischemia: Reperfusion in mice

M Zamani, J Hassanshahi^,, M Soleimani, F Zamani

Department of Anatomy, Esfahan University of Medical Sciences, Esfahan, Iran

1 Department of Physiology, Esfahan University of Medical Sciences, Esfahan, Iran

2 Department of Histology, Tehran University of Medical Sciences, Tehran, Iran

3 Department of Nursing, School of Nursing, Esfahan University of Medical Sciences, Esfahan, Iran

Dr. Hassanshahi J Department of physiology, Tehran University of Medical Sciences Tehran Iran hasanshahij@gmail.com

Journal of Neurosciences in Rural Practice

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This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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This article was originally published by Thieme Medical and Scientific Publishers Private Ltd. and was migrated to Scientific Scholar after the change of Publisher.

Abstract

ABSTRACT

Introduction: Transient global ischemia induces selective, delayed neuronal death of pyramidal neurons in the hippocampal CA1. Oxidative Stress is considered to be involved in a number of human diseases including ischemia. Preliminary studies confirmed reduction of cell death in brain following treatment with antioxidants. Aim: According to this finding, we study the relationship between consumption of olive oil on cell death and memory disorder in brain ischemia. We studied the protective effect of olive oil against ischemia‑reperfusion. Material and Methods: Experimental design includes three groups: Intact (n = 8), ischemic control (n = 8) and treatment groups with olive oil (n = 8). The mice treated with olive oil as pre‑treatment for a week. Then, ischemia induced by common carotid artery ligation and following the reduction of inflammation [a week after ischemia], the mice post‑treated with olive oil. Nissl staining applied for counting necrotic cells in hippocampus CA1. Tunnel kit was used to quantify apoptotic cell death while to short term memory scale, we apply y‑maze and shuttle box tests and for detection the rate of apoptotic and treated cell, we used western blotting test for bax and bcl2 proteins. Results: High rate of apoptosis was seen in ischemic group that significantly associated with short‑term memory loss. Cell death was significantly lower when mice treated with olive oil. The memory test results were adjusted with cell death results and bax and bcl2 expression in all groups’ comparison. Ischemia for 15 min induced cell death in hippocampus with more potent effect on CA1. Conclusion: Olive oil intake significantly reduced cell death and decreased memory loss.

Keywords

Antioxidant

hippocampus CA1

ischemia‑reperfusion

neuroprotective effect

olive oil

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Conflict of Interest

None declared

References

Paul C. M., Magda G, Abel S, . Spatial memory: Theoretical basis and comparative review on experimental methods in rodents. Behave Brain Res. 2009;203:151-64.
[Google Scholar]
Gavin A Scott, Mbongeni Mtetwa, Hugo Lehmann, . Novel odour recognition memory is independent of the hippocampus in rats. Experimental Brain Research 224.2. 2013:199-209.
[Google Scholar]
O’Keefe J, Dostrovsky J, . The hippocampus as a spatial map. Preliminary evidence from unit activityin the freely moving rat. Brain Res. 1971;34:171-5.
[Google Scholar]
Gorden M. S., . Neurobiology. (3 rd ed). United Kingdom: Oxford University Press; 2000. p. :p. 34-618.
[Google Scholar]
Behnam M, . Cellol haie asabi vasystem haie asabi. Iran: Ferdowsi University of Mashhad; 1996.
[Google Scholar]
Lee H, Ghim J. W., Kim H, Lee D, Jung M, . Hippocampal neural correlates for values of experienced events. J Neurosci. 2012;32:15053-65.
[Google Scholar]
Albasser M. M., Amin E, Lin T. C., Iordanova M. D., Aggleton J. P., . Evidence that the rat hippocampus has contrasting roles in object recognition memory and object recency memory. Behav Neurosci. 2012;126:659-69.
[Google Scholar]
Herrick J, . Clinical features of sudden obstruction of the coronary arteries. JAMA. 1912;59:2015-20.
[Google Scholar]
Bernard S. A., Gray T. W., Buist M. D., Jones B. M., Silvester W, Gutteridge G, et al . Treatment of comatose survivors of out-ofhospitalcardiac arrest with induced hypothermia. N Engl J Med. 2002;346:557-63.
[Google Scholar]
Doyle K. P., Simon R. P., Stenzel-poore M. P., . Mechanism of ischemic brain damage. Neuropharmaco. 2008;55:310-8.
[Google Scholar]
Sahay A, Hen R, . Adult hippocampal neurogenesis in depression. Nat Neurosci. 2007;10:1110-5.
[Google Scholar]
Huang L, Chen N, Ge M, Zhu Y, Guan S, Wang J. H., . Ca2+and acidosis synergistically lead to the dysfunction of cortical GABAergic neurons during ischemia. Biochem Biophys Res Commun. 2010;394:709-14.
[Google Scholar]
Weglicki W. B., Dickens B. F., Mak I. T., . Enhanced lysosomal phospholipids degradation and lysosphospholipid production due to free radicals. Biochem Biophys Res Commun. 1984;124:229-35.
[Google Scholar]
Kesner R. P., Crutcher K, Beers D. R., . Serial position curves for item (spatial location) information: role of dorsal hippocampal formation and medial septum. Brain Res. 1988;454:219-26.
[Google Scholar]
Perez-Jimenez F, Alvarez de Cienfuegos G, Badimon L, Barja G, Battino M, Blanco A, et al . Visioli F, International conference on the healthy effect of virginolive oil. Eur J Clin Invest. 2005;35:421-4.
[Google Scholar]
Harrison D, Griendling K. K., Landmesser U, Hornig B, Drexler H, . Role of oxidative stress in atherosclerosis. Am J Cadiol. 2003;40:7-11.
[Google Scholar]
Mirmiran P, Esmaillzadeh A, Azizi F, . Detection of cardiovascular risk factors by anthropometri measures in Tehran Iran adults: Receiver operating characteristic (ROC) curve analysis. Eur J clin Nutr. 2004;58:1110-8.
[Google Scholar]
Hayakawa T, Waltz A. G., . Immediate effects of cerebral ischemia: Evaluation and resolution of neurological deficits after experimental occlusion of one middle cerebral artery in conscious cats. Stroke. 1975;6:321-7.
[Google Scholar]
de la Puerta R, Martínez Domínguez M. E., Ruíz-Gutíerrez V, Flavill J. A., Hoult J. R., . Effects of virgin olive oil on scavenging of reactive nitrogen species and upon nitrergic neurotransmission. Life Sci. 2001;69:1213-22.
[Google Scholar]
Allman-Farinelli M. A., Gomes K, Favaloro E. J., Petocz P, . A diet rich in high-oleic-acid sunflower oil favorably alters low-density lipoprotein cholesterol, triglycerides, and factor VII coagulant activity. J Am Diet Assoc. 2005;105:1071-9.
[Google Scholar]
Paknahad Z, Mahdavi R, Asgari S, Mahboub S, Rajabi P, Abadeh K. N., . Comparison of the effect of high olive oil/hydrogenated oil diet on serum lipoproteinsand atherosclerosis formation in rabbit. Tehran, Iran: Proceedings ofthe second congress of prevention of the non communicable diseases; 2004. p. :p. 188.
[Google Scholar]
Bandyopadhyay I, Saha S, Dutta J, . Effect of dietary fish oil on platelet aggregibility: Comparison between two oils with different eicosapentaenoic to arachidonic acid ratio. Biochem Int. 1991;25:919-28.
[Google Scholar]
Cho J, Kang J. S., Long P. H., Jing J, Back Y, Chung K. S., . Antioxidant and memory enhancing effects of purple sweet potato anthocyaninand cordyceps mushroom extract. Arch Pharm Res. 2003;26:821-5.
[Google Scholar]
Hasselmo M. E., . The role of acetylcholine in learning and memory. Curr Opin Neurobiol. 2006;16:710-5.
[Google Scholar]
Lee L, Kang S. A., Lee H. O., Lee B. H., Jung I. K., Lee J. E., et al . Effect of supplementation of vitamin E and vitamin C on brain acetyl cholinesterase activity and neurotransmitter levels in rats treated with scopolamine, an inducer of dementia. J Nutr Sc Vitaminol (Tokyo). 2001;47:323-8.
[Google Scholar]
Violi F, Cangemi R, . Antioxidants and cardiovascular disease. Curr Opin Investig Drugs. 2005;6:895-900.
[Google Scholar]
Visioli F, Poli A, Galli C, . Antioxidant and other biological activities of phenols from olives and olive oil. Med Res Rev. 2002;22:65-75.
[Google Scholar]

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[1] Paul C. M., Magda G, Abel S, . Spatial memory: Theoretical basis and comparative review on experimental methods in rodents. Behave Brain Res. 2009;203:151-64.
[Google Scholar]

[2] Gavin A Scott, Mbongeni Mtetwa, Hugo Lehmann, . Novel odour recognition memory is independent of the hippocampus in rats. Experimental Brain Research 224.2. 2013:199-209.
[Google Scholar]

[3] O’Keefe J, Dostrovsky J, . The hippocampus as a spatial map. Preliminary evidence from unit activityin the freely moving rat. Brain Res. 1971;34:171-5.
[Google Scholar]

[4] Gorden M. S., . Neurobiology. (3 rd ed). United Kingdom: Oxford University Press; 2000. p. :p. 34-618.
[Google Scholar]

[5] Behnam M, . Cellol haie asabi vasystem haie asabi. Iran: Ferdowsi University of Mashhad; 1996.
[Google Scholar]

[6] Lee H, Ghim J. W., Kim H, Lee D, Jung M, . Hippocampal neural correlates for values of experienced events. J Neurosci. 2012;32:15053-65.
[Google Scholar]

[7] Albasser M. M., Amin E, Lin T. C., Iordanova M. D., Aggleton J. P., . Evidence that the rat hippocampus has contrasting roles in object recognition memory and object recency memory. Behav Neurosci. 2012;126:659-69.
[Google Scholar]

[8] Herrick J, . Clinical features of sudden obstruction of the coronary arteries. JAMA. 1912;59:2015-20.
[Google Scholar]

[9] Bernard S. A., Gray T. W., Buist M. D., Jones B. M., Silvester W, Gutteridge G, et al . Treatment of comatose survivors of out-ofhospitalcardiac arrest with induced hypothermia. N Engl J Med. 2002;346:557-63.
[Google Scholar]

[10] Doyle K. P., Simon R. P., Stenzel-poore M. P., . Mechanism of ischemic brain damage. Neuropharmaco. 2008;55:310-8.
[Google Scholar]

[11] Sahay A, Hen R, . Adult hippocampal neurogenesis in depression. Nat Neurosci. 2007;10:1110-5.
[Google Scholar]

[12] Huang L, Chen N, Ge M, Zhu Y, Guan S, Wang J. H., . Ca2+and acidosis synergistically lead to the dysfunction of cortical GABAergic neurons during ischemia. Biochem Biophys Res Commun. 2010;394:709-14.
[Google Scholar]

[13] Weglicki W. B., Dickens B. F., Mak I. T., . Enhanced lysosomal phospholipids degradation and lysosphospholipid production due to free radicals. Biochem Biophys Res Commun. 1984;124:229-35.
[Google Scholar]

[14] Kesner R. P., Crutcher K, Beers D. R., . Serial position curves for item (spatial location) information: role of dorsal hippocampal formation and medial septum. Brain Res. 1988;454:219-26.
[Google Scholar]

[15] Perez-Jimenez F, Alvarez de Cienfuegos G, Badimon L, Barja G, Battino M, Blanco A, et al . Visioli F, International conference on the healthy effect of virginolive oil. Eur J Clin Invest. 2005;35:421-4.
[Google Scholar]

[16] Harrison D, Griendling K. K., Landmesser U, Hornig B, Drexler H, . Role of oxidative stress in atherosclerosis. Am J Cadiol. 2003;40:7-11.
[Google Scholar]

[17] Mirmiran P, Esmaillzadeh A, Azizi F, . Detection of cardiovascular risk factors by anthropometri measures in Tehran Iran adults: Receiver operating characteristic (ROC) curve analysis. Eur J clin Nutr. 2004;58:1110-8.
[Google Scholar]

[18] Hayakawa T, Waltz A. G., . Immediate effects of cerebral ischemia: Evaluation and resolution of neurological deficits after experimental occlusion of one middle cerebral artery in conscious cats. Stroke. 1975;6:321-7.
[Google Scholar]

[19] de la Puerta R, Martínez Domínguez M. E., Ruíz-Gutíerrez V, Flavill J. A., Hoult J. R., . Effects of virgin olive oil on scavenging of reactive nitrogen species and upon nitrergic neurotransmission. Life Sci. 2001;69:1213-22.
[Google Scholar]

[20] Allman-Farinelli M. A., Gomes K, Favaloro E. J., Petocz P, . A diet rich in high-oleic-acid sunflower oil favorably alters low-density lipoprotein cholesterol, triglycerides, and factor VII coagulant activity. J Am Diet Assoc. 2005;105:1071-9.
[Google Scholar]

[21] Paknahad Z, Mahdavi R, Asgari S, Mahboub S, Rajabi P, Abadeh K. N., . Comparison of the effect of high olive oil/hydrogenated oil diet on serum lipoproteinsand atherosclerosis formation in rabbit. Tehran, Iran: Proceedings ofthe second congress of prevention of the non communicable diseases; 2004. p. :p. 188.
[Google Scholar]

[22] Bandyopadhyay I, Saha S, Dutta J, . Effect of dietary fish oil on platelet aggregibility: Comparison between two oils with different eicosapentaenoic to arachidonic acid ratio. Biochem Int. 1991;25:919-28.
[Google Scholar]

[23] Cho J, Kang J. S., Long P. H., Jing J, Back Y, Chung K. S., . Antioxidant and memory enhancing effects of purple sweet potato anthocyaninand cordyceps mushroom extract. Arch Pharm Res. 2003;26:821-5.
[Google Scholar]

[24] Hasselmo M. E., . The role of acetylcholine in learning and memory. Curr Opin Neurobiol. 2006;16:710-5.
[Google Scholar]

[25] Lee L, Kang S. A., Lee H. O., Lee B. H., Jung I. K., Lee J. E., et al . Effect of supplementation of vitamin E and vitamin C on brain acetyl cholinesterase activity and neurotransmitter levels in rats treated with scopolamine, an inducer of dementia. J Nutr Sc Vitaminol (Tokyo). 2001;47:323-8.
[Google Scholar]

[26] Violi F, Cangemi R, . Antioxidants and cardiovascular disease. Curr Opin Investig Drugs. 2005;6:895-900.
[Google Scholar]

[27] Visioli F, Poli A, Galli C, . Antioxidant and other biological activities of phenols from olives and olive oil. Med Res Rev. 2002;22:65-75.
[Google Scholar]

Neuroprotective effect of olive oil in the hippocampus CA1 neurons following ischemia: Reperfusion in mice

Abstract

ABSTRACT

Keywords

Antioxidant

hippocampus CA1

ischemia‑reperfusion

neuroprotective effect

olive oil

Conflict of Interest

References

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